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NUITFRANCE - Bibliothèque - Fiche bibliographique
Bibliothèque
Cette rubrique recense :
- de la documentation sur les différents thèmes de la nuit (vie nocturne, pollution lumineuse, pollution sonore, ...).
- les données informatiques relatives à l'éclairage public digitalisées et mises à dispositions en open data par certaines communes,
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Quelques tags associés : [ DOCUMENTATION, PUBLICATIONS, LITTÉRATURE, CONNAISSANCES, LITTÉRATURE GRISE, ARTICLES DE PRESSE, ARTICLES SCIENTIFIQUES, TEXTES JURIDIQUES, PLANS ET PROGRAMMES, JURISPRUDENCE, DÉCRETS, THÈSES ]
► Fiche bibliographique
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Document " Effects of light-emitting diode radiations on human retinal pigment epithelial cells in vitro "
Type de document : |
Articles de revue scientifique |
Thème du document : |
Nuit menacée - Lumière artificielle - Impacts sur l'Homme (santé, sommeil, vieillissement) |
Groupe biologique : |
Etres humains |
Auteur(s) : |
CHAMORRO E. BONNIN-ARIAS C. PÉREZ-CARRASCO M.J. MUÑOZ DE LUNA J. VÁZQUEZ D. SÁNCHEZ-RAMOS C.
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Date de publication : |
Mars 2013 |
Langue : |
English/Anglais |
Nom du périodique : |
Photochemistry and Photobiology |
Précisions : |
Volume 89. Numéro 3. Pages 468-473 |
Lien contenu/source : |
http://www.ncbi.nlm.nih.gov/pubmed/22989198 |
DOI : |
10.1111/j.1751-1097.2012.01237.x |
Citation courte : |
Chamorro et al. (2013) |
Citation complète (format NuitFrance) : |
CHAMORRO E., BONNIN-ARIAS C., PÉREZ-CARRASCO M.J., MUÑOZ DE LUNA J., VÁZQUEZ D. & SÁNCHEZ-RAMOS C. (2013). Effects of light-emitting diode radiations on human retinal pigment epithelial cells in vitro. Photochemistry and Photobiology. Volume 89. Numéro 3. Pages 468-473. |
Résumé du document : |
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Human visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.
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Saisie sur NuitFrance par : |
Rosor |
Saisie sur NuitFrance en : |
Novembre 2014 |
Identifiant NuitFrance : |
NF-BIBLI-984 |
Permalien de la fiche NuitFrance : |
http://www.nuitfrance.fr/?page=donneesdoc&partie=fiche-bibliographique&id_doc=984 |
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