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NUITFRANCE - Bibliothèque - Fiche bibliographique
Bibliothèque
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Document " Early changes in gene expression induced by blue light irradiation of A2E-laden retinal pigment epithelial cells "
Type de document : |
Articles de revue scientifique |
Thème du document : |
Nuit menacée - Lumière artificielle - Impacts sur l'Homme (santé, sommeil, vieillissement) |
Groupe biologique : |
Etres humains |
Auteur(s) : |
VAN DER BURGHT B.W. HANSEN M. OLSEN J. ZHOU J. WU Y. NISSEN M.H. SPARROW J.R.
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Date de publication : |
Novembre 2013 |
Langue : |
English/Anglais |
Nom du périodique : |
Acta Ophthalmologica |
Précisions : |
Volume 91. Numéro 7. Pages e537–e545 |
Lien contenu/source : |
http://onlinelibrary.wiley.com/doi/10.1111/aos.121... |
DOI : |
10.1111/aos.12146 |
Mots-clefs : |
A2E Age-related macular degeneration Apoptosis Complement cascade Gene expression Retinal pigment epithelial cells
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Citation courte : |
Van der burght et al. (2013) |
Citation complète (format NuitFrance) : |
VAN DER BURGHT B.W., HANSEN M., OLSEN J., ZHOU J., WU Y., NISSEN M.H. & SPARROW J.R. (2013). Early changes in gene expression induced by blue light irradiation of A2E-laden retinal pigment epithelial cells. Acta Ophthalmologica. Volume 91. Numéro 7. Pages e537–e545. |
Résumé du document : |
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Purpose: Accumulation of bisretinoids as lipofuscin in retinal pigment epithelial (RPE) cells is implicated in the pathogenesis of some blinding diseases including age-related macular degeneration (AMD). To identify genes whose expression may change under conditions of bisretinoid accumulation, we investigated the differential gene expression in RPE cells that had accumulated the lipofuscin fluorophore A2E and were exposed to blue light (430 nm).
Methods: A2E-laden RPE cells were exposed to blue light (A2E/430 nm) at various time intervals. Cell death was quantified using Dead Red staining, and RNA levels for the entire genome was determined using DNA microarrays (Affymetrix GeneChip Human Genome 2.0 Plus). Array results for selected genes were confirmed by real-time reverse-transcriptase polymerase chain reaction.
Results: Principal component analysis revealed that the A2E-laden RPE cells irradiated with blue light were clearly distinguishable from the control samples. We found differential regulation of genes belonging to the following functional groups: transcription factors, stress response, apoptosis and immune response. Among the last mentioned were downregulation of four genes that coded for proteins that have an inhibitory effect on the complement cascade: (complement factor H, complement factor H-related 1, complement factor I and vitronectin) and of two belonging to the classical pathway (complement component 1, s subcomponent and complement component 1, r subcomponent).
Conclusion: This study demonstrates that blue light irradiation of A2E-laden RPE cells can alter the transcription of genes belonging to different functional pathways including stress response, apoptosis and the immune response. We suggest that these molecules may be associated to the pathogenesis of AMD and can potentially serve as future therapeutic targets.
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Saisie sur NuitFrance par : |
Rosor |
Saisie sur NuitFrance en : |
Octobre 2014 |
Identifiant NuitFrance : |
NF-BIBLI-804 |
Permalien de la fiche NuitFrance : |
http://www.nuitfrance.fr/?page=donneesdoc&partie=fiche-bibliographique&id_doc=804 |
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